3USS was developed to automatically identify alternative 3’UTRs (shorter or longer with respect to
a reference transcriptome) in RNA-seq experiments. In addition, the tool tries to distinguish putative
novel (not already known) 3’UTRs, comparing them to the annotated ones in various databases.
When a user uploads data from two samples, their alternative 3’UTRs are compared, highligthing those shared in diverse cell types or occurring in specific biological conditions.
Although the tool was thought for managing RNA-seq assembled transcripts, it provides another utility that aids to easily retrieve 3’UTR genomic coordinates and nucleotide sequences of already annotated transcripts.